Journal: Cell reports

Article Title: Chromosome silencing in vitro reveals trisomy 21 causes cell-autonomous deficits in angiogenesis and early dysregulation in Notch signaling

doi: 10.1016/j.celrep.2022.111174

Figure Lengend Snippet:

Article Snippet: anti-vWF (rabbit polyclonal) , Sino Biological Inc , Cat#: 10973-T26; RRID: AB_2860257.

Techniques: Recombinant, Blocking Assay, Multiplex Assay, Isolation, Transgenic Assay, Software

Journal: Critical Care

Article Title: Early release of high mobility group box nuclear protein 1 after severe trauma in humans: role of injury severity and tissue hypoperfusion

doi: 10.1186/cc8152

Figure Lengend Snippet: High plasma levels of HMGB1 are associated with the release of inflammatory mediators and markers of endothelial cell and complement activation in trauma patients. Blood samples were obtained from 168 consecutive major trauma patients immediately upon admission to the hospital. (a to d) Plasma levels of high mobility group box nuclear protein 1 (HMGB1) are associated with increased plasma levels of IL-6, TNF-α, Von Willebrand Factor (vWF) and angiopoietin-2 (Ang-2). (e) High plasma levels of HMGB1 are associated with increased complement activity as indicated by elevated soluble C5b-9 plasma levels that are generated during the late phase of complement activation. Data are presented in quartiles, * P ≤ 0.05 based on test for rank and trend.

Article Snippet: These results were compared with IL-6, TNF-α (both from R&D Systems Inc., Minneapolis, MN, USA), von Willebrand Factor (vWF) antigen (Asserachrom vWF, Diagnostica Stago Inc., Parsippany, NJ, USA), angiopoietin-2 (Ang-2; Quantikine Ang-2 EIA, R&D Systems Inc., Minneapolis, MN, USA), soluble C5b-9 to assess the late phase of terminal complement activation (sC5b-9 EIA, Quidel Corp., San Diego, CA, USA), prothrombin fragments (PF 1+2; Enzygnost F1+2 EIA, Dade Behring, Germany), soluble thrombomodulin (TM; Asserachrom Thrombomodulin EIA, Diagnostica Stago Inc., Parsippany, NJ, USA), and plasminogen activator inhibitor 1 (PAI-1; Oxford Biochemicals, Oxford, MI, US). protein C activity, tissue plasminogen activator (t-PA), and D-Dimers were measured with a Stago Compact (Diagnostica Stago Inc., Parsippany, NJ, USA), All measurements were performed in accordance with the manufacturers' instructions.

Techniques: Activation Assay, Activity Assay, Generated